Stabilization of the nanoparticles in BSA solution results in a stable dispersion without sedimentation with a ZP value around −32 mV for 1 mg/mL of nanoparticle concentration. The produced nanoparticles demonstrate ferromagnetic behavior with the saturation magnetization value of M s = 83.6 emu/g. The obtained nanoparticles feature a mean diameter of 15 ± 4 nm with a shell thickness of 2.5 ± 0.6 nm. core–shell nanoparticles were synthesized by the flow–levitation method. It might be due to the fact that the blocking temperatures of the smaller particles are near 80 K, while their magnetic moments are blocked below 80 K, and also with being the formation of the spin glass state for Fe 3C at 75K. On the other hand, magnetization decreases faster below 80 K. The M ZFC curve indicates an incomplete maximum within the measurement’s temperature limit, meaning that blocking temperature (TB) lies above 300 K at the applied field of 100 Oe. By the end of the cooling process, the ferromagnetic moments remain blocked in the direction of the applied field. Further increase in temperature leads to an increase in the net magnetization due to the alignment of the ferromagnetic moments in the direction of the magnetic field. This behavior can be explained by thermally activated ferromagnetic moments of Pre-cooling the sample to 2 K leads to a chaotic distribution of magnetic moments. Temperature dependences of the zero-field-cooling and field-cooling magnetizations (M ZFC and M FC, respectively), in the sample in the whole temperature range of 2–300 K with splitting between M FC and M ZFC below 300 K are given in ( Figure 3b). The high SAR depends on numerous parameters of NPs: size, shape, composition, magnetic interaction, concentration, as well as the applied magnetic field frequency and strength. Specific absorption rate (SAR) is a key performance parameter marking the applicability of a material and determining the dose and duration of the MHT. Hyperthermia also amplifies the immune responses in the body against cancer while decreasing the immune suppression and immune escape of cancer. MHT employs the heat generated by a magnetic nanoparticle subjected to an alternating magnetic field the heat, consequently, induces local cytotoxic effects such as denaturation of cytoplasmic and membrane tumor proteins, reduction in blood flow, and induction of tumor acidosis. Nevertheless, magnetic hyperthermia therapy (MHT), a complementary cancer treatment method, is one of the most promising techniques. Magnetic nanoparticles (NPs) are now actively applied in various technological fields, including data storage, catalysis, MRI contrast agents, and drug delivery.